I usually find a sample a few semitones off, or exactly in the key I want. Also, going a 5th above the root note is okay too, especially if you're using a powerful bass.
I usually drag my sample into a drum rack in ableton, then throw a spectrum on it, and see where the lowest peaking frequency is, for example a kick in E will be around 41hz. If you cant quite get it off of that, then transpose the kick sample up an octave, and its usually easier to tell.
If that doesn't work, or its still unclear, throw an EQ on, cut away all the higher frequencies, and play a sine wave alongside the kick.