It really depends on the type of kick. There are three basic types of kick sounds in EDM -- at least this is how I categorize them.
1. The tonal kind, with a long tail and a clear sub note (like a distorted 808 kick)
2. The more noisy kind, covering a large frequency with no clear note (like some acoustic kicks)
3. The ones with a really strong pitch envelope, that almost sound like a "laser zap" noise (like a lot of classic drum machine kicks)
Some EDM genres use one particular type. In house and trance I've heard all three types.
Type 1 has a clear note, so you need to be aware of which note it's playing. It doesn't have to be the root note but you probably don't want a super-dissonant tone.
Type 2 usually doesn't have a recognizable note. If your ears or a tuner can't find a note, this is probably the case. In this case, it's probably not necessary to tune it. (This is why rock bands don't worry about tuning kicks).
Type 3 can have a recognizable pitch, depending on how fast the pitch envelope is. You might have to tune those too.